Determination of aluminium and FLOURIDES in food

DETERMINATION OF ALUMINIUM AND FLUORIDE LEVELS IN SOME BRANDS OF INDUSTRIALLY MADE COMMERCIAL FLOUR

BY
AMEH FRIDAY
REG. NO. FPI/HND/SLT/011/002

BEING A PROJECT SUBMITTED TO THE DEPARTMENT OF
SCIENCE LABORATORY TECHNOLOGY
FEDERAL POLYTECHNIC IDAH

IN PARTIAL FULFILMENT OF THE REQUIREMENT FOR
THE AWARD OF
HIGHER NATIONAL DIPLOMA (HND) CERTIFICATE IN
SCIENCE LABORATORY TECHNOLOGY
(CHEMISTRY/BIOCHEMISTRY OPTION)
FEDERAL POLYTECHNIC IDAH, KOGI STATE.

DECEMBER, 2013
CERTIFICATION
This project work entitled determination of aluminium and fluoride level in some brands of industrially made commercial flour by AMEH FRIDAY with Reg. No. FPI/HND/SLT/011/002 meets part of the regulation governing the award of Higher National Diploma in the department of Science Laboratory Technology, Federal Polytechnic Idah and it is approved for it's contribution to knowledge and literary presentation.

_____________________ ___________________
Mrs. O. A. UPU DATE
(SUPERVISOR)

_____________________ ____________________
Mallam I. O. UMAR DATE
(H.O.D)

_____________________ _____________________
EXTERNAL SUPERVISOR DATE

DEDICATION
This project work is dedicated to God the Father, the Son and the Holy Spirit and to the lovely family Godgave to me that is the family of Mr. Ameh, Gabriel Ochegbe.

ACKNOWLEDGEMENT
My appreciation and thanks goes to Almighty God for His guidiance and unmerited favour over my life and throughout my program in the Federal Polytechnic Idah.
I am sincerely grateful to my supervisor Mrs. O. A UPU who has devoted her time and resources to make sure this project is successful.
My gratitude goes to my head of department Mallam I. OUmar and all the lecturers that has imparted discipline and knowledge in me.
My immeasurable thanks goes to my beloved mother for standing beside me to make sure am successful in life, my sisters and to my uncles especially Mr. Onuche Yakubu for his unmerited love and support for me.
Finally my appreciation goes to all my course mates, friends and loved ones that help me to complete my Higher National Diploma (HND) program.

ABSTRACT
Industrially made commercial flour mostly used as staple food by man were analyzedfor the level of aluminium and fluoride. All the nine samples analyzed contains aluminium indicating that the manufacturers used aluminium compounds as anticaking agent. The value obtained range from 5.21 to 8.79mg/kg of sample. Most of the samples contained fluoride in the range of 0.05 to 0.08mg/kg of sample, except two out of the nine samples that the fluoride levels were not detected. Although, levels of aluminium obtained are within the limit stated by FAO and WHO standard, it is recommended that alternative additives be used in place of aluminium compounds.

TABLE OF CONTENT
TITLE PAGE...............................................................................................i
CERTIFICATION ......................................................................................ii
DEDICATION ............................................................................................iii
ACKNOWLEDGMENT...........................................................................iv
ABSTRACT................................................................................................ v
TABLE OF CONTENTS...................................................................................vi
CHAPTER ONE
1.0 INTRODUCTION.................................................................................1
1.1 AIMS AND OBJECTIVES...................................................................... 2
CHAPTER TWO
2.0 LITERATURE REVIEW.......................................................................... 3
2.1 FOOD ADDITIVES............................................................................... 3
2.2 COMMONLY USED F
CHAPTER THREE
3.0 MATERIALS AND METHODS...................................................... 13
3.1 STUDY LOCATION........................................................................ 13
3.2 SAMPLE COLLECTION AND STORAGE ................................... 13
3.3 EXPERIMENTAL DESIGN............................................................ 13
3.4 ALUMINIUM DETERMINATION ............................................... 14
3.4.1 INSTRUMENTS.............................................................................. 14
3.4.2 REAGENTS..................................................................................... 14
3.4.3 SAMPLE PREPARATION............................................................. 14
3.4.4 PRECONCENTRATION PROCEDURE....................................... 15
3.5 FLUORIDE DETERMINATION ................................................... 15
3.5.1 REAGENTS AND APPARATUS................................................... 15
3.5.2 REAGENTS USED......................................................................... 16
3.5.3 SAMPLE PREPARATION............................................................. 16
3.5.4 SPECTROMETRIC DETERMINATION OF FLUORIDE............ 16
CHAPTER FOUR
4.0 RESULT AND DISCUSSION............................................................ 20
CHAPTER FIVE
5.1 CONCLUSSION AND RECOMMENDATION................................ 22
REFERENCES.......................................................................................... 23
APPENDIXES........................................................................................... 29